Journal: eLife
Article Title: SPAG7 deletion causes intrauterine growth restriction, resulting in adulthood obesity and metabolic dysfunction
doi: 10.7554/eLife.91114
Figure Lengend Snippet: ( A ) Cumulative food intake as determined by BioDaq Food and Water intake monitoring system. N=7. ( B ) Hourly energy expenditure as determined by CLAMS metabolic cage system. N=7. ( C ) Total energy expenditure as determined by CLAMS metabolic cage system. n=7. Significance was assessed by Welch’s two sample t-test. ( D ) Home cage locomotor activity as determined by CLAMS metabolic cage system. n=7. Significance was assessed by Welch’s two sample t-test. ( E ) Body weight over time of WT vs SPAG7 KO animals raised at thermoneutrality. n=7. Significance was assessed by Welch’s two sample t-test. ( F ) Fat mass over time of animals at thermoneutrality. n=7. Significance was assessed by Welch’s two sample t-test. ( G ) Daily food intake of animals at thermoneutrality. N=7. ( H ) Total energy expenditure of animals at thermoneutrality as determined by CLAMS. n=7. Significance was assessed by Welch’s two sample t-test. ( I ) Body weight over time of WT vs SPAG7 KO animals raised on high-fat diet. n=8. Significance was assessed by Welch’s two sample t-test.( J ) Fat mass over time of animals fed high-fat diet. n=8. Significance was assessed by Welch’s two sample t-test. ( K ) Daily food intake of animals fed high-fat diet. N=8. ( L ) Total energy expenditure of animals fed high-fat diet. n=8. Significance was assessed by Welch’s two sample t-test. ( M ) Percent body weight difference in SPAG7 KO animals vs WT fed chow diet at room temperature (Chow), fed chow diet at thermoneutrality (TN), or HFD at room temperature (HFD) at 20 weeks of age. n=7. * p<0.05, ** p<0.01.
Article Snippet: Food intake was further monitored using the BioDAQ food and water intake monitoring system system (Research Diets, New Brunswick, NJ).
Techniques: Activity Assay
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